A full factorial design 2 3 with 1 center point and 2 replicates was used to identify the factors that have significant impacts on biomass, biosurfactant and lipase production in SmgB. Simultaneously obtaining compounds in a singular bioprocess would result in higher process efficiencies. It can be clearly seen Table 1 that the addition of soybean oil into the culture medium stimulated the biomass production assays 5, 6, 7, 8 and 9. Sarkar and Laha obtained higher lipase production values when using glucose and olive oil as substrates and reduced lipolytic activity values were observed when using glucose as the sole carbon source. Biomass quantification Biomass quantification in the culture medium of SmgB was determined by the dry mass technique. Similar values for biomass were observed by Colla et al. Simultaneous production of lipases and biosurfactants by submerged and solid-state bioprocesses, Bioresource Technology, , p. It is also known that emulsification activities from Aspergillus strains strongly correlate with the production of biosurfactants Castiglioni et al.
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